This test is used to assist in the identification of Pseudomonas, Neisseria, Alcaligens, Aeromonas, Campylobacter, Vibrio, Brucella and Pasteurella, all of which produce the enzyme cytochrome oxidase. A number of reagents can be used for this test. Kovacs Oxidase Reagent: 1% tetra-methyl- p -phenylenediamine dihydrochloride, in water.