Comparison between direct smear, formalin-ethyl acetate concentration, and Mini Parasep® Solvent-Free Concentrator for screening of intestinal parasitic …

In an effort to improve the standard of care, the comparative accuracy in detecting helminth infections of the hitherto used formalin-based concentration method (FC) was compared to a previously developed formalin ethyl-acetate-based concentration technique (FECT), prior to the systematic deployment of the latter at a research and …

Aims: The objective was to compare the sedimentation spontaneous in tube technique (SSTT), the direct smear, the zinc sulfhate (surface film and sediment analyzed) methods with the formalin-ethyl ...

This new procedure of centrifugal-sedimentation of fecal samples for intestinal parasite research was then reported as formalin-ethyl acetate sedimentation concentration technique 23. The performance of the centrifugal-sedimentation processes compared with other techniques/methods was reported by Yanet et al.

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered.

Comparison of a Urine Antigen Assay and Multiple Examinations with the Formalin-Ethyl Acetate Concentration Technique for Diagnosis of Opisthorchiasis Am J Trop Med Hyg. 2023 ... (2.5%). In comparison with the composite reference standard (combined FECT and urine antigen detection), the diagnostic sensitivity of examining two …

Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques for detection of intestinal parasites. J Clin Microbiol. 1981 May; 13 (5):882–884. [PMC free article] [Google Scholar] Young KH, Bullock SL, Melvin DM, Spruill CL. Ethyl acetate as a substitute for diethyl ether in the ...

Su et al. found that formalin-based fixation is preferable to compare to ethanol 99% in analysing cell morphology. Gong et al., demonstrated significantly lower detection rates of Ki-67, PCNA, and p53 with the ethanol-based fixative ThinPrep as compared with formalin-fixed cell-block slides in malignant cases . This is consistent …

The quantitative formalin ethyl acetate concentration technique (QFEC) was compared to agar plate culture () for the detection of Strongyloides stercoralis larvae. QFEC could substitute for only when the parasite …

The quantitative formalin ethyl acetate concentration technique (QFEC) was compared to agar plate culture () for the detection of Strongyloides …

Sanprasert V Charuchaibovorn S Bunkasem U Srirungruang S Nuchprayoon S. Comparison between direct smear, formalin-ethyl acetate concentration, and mini Parasep® solvent-Free concentrator for screening of intestinal parasitic infections among school-age children Chulalongkorn Med J 60 2016 256 269 Sanprasert V, …

Clinical comparison of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique. Author: D D Erdman Authors Info & Affiliations. DOI: …

Hence, there is a need for a low-complexity, high-throughput, and cost-effective alternative to labor-intensive microscopic examinations. This study aimed to compare the performance of a fully automatic digital feces analyzer, Orienter Model FA280 (People's Republic of China) with that of the formalin-ethyl acetate concentration …

This chapter discusses about macroscopic examination and microscopic examination of fecal specimens. The microscopic examination of the stool specimen, normally called the ova and parasite examination, consists of three separate techniques: the direct wet smear, the concentration (sedimentation and flotation), and the permanent …

Abstract. A substitute for the volatile solvent diethyl ether has been actively sought for the Formalin-ether sedimentation technique. Ethyl acetate has recently been shown to be a comparable substitute. In an effort to verify these findings and evaluate ethyl acetate under clinical conditions, comparison studies with 62 fresh human stool ...

Comparison between direct smear, formalin-ethyl acetate concentration, and Mini Parasep® Solvent-Free Concentrator for screening of intestinal parasitic infections among school-age children.

Formalin-ethyl acetate concentration technique (FECT) Fecal examination using a modified FECT diagnostic method was conducted as reported previously [23, 24]. Briefly, fecal samples were homogenized, and 2 g of fresh feces was weighed and diluted in 7 ml of 10% formalin solution and strained through two layers of gauze into the 15-ml …

Comparison between direct smear, formalin-ethyl acetate concentration, and Mini Parasep® Solvent-Free Concentrator for screening of intestinal parasitic infections among school-age children ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and …

Briefly, the preserved fecal suspension was filtered through two layers of wet gauze into a centrifuge tube. The volume was adjusted to 10 ml with 10% formalin. Two milliliters of ethyl acetate was added, and the tube was closed and shaken for half a second. The stopper was removed, and the tube was centrifuged at 600 × g for 10 min. The plug ...

Comparison between direct smear, formalin-ethyl acetate concentration, and Mini Parasep® Solvent-Free Concentrator for screening of intestinal parasitic infections among school-age children ...

Formalin-ethyl acetate concentration technique (FECT) is the method used to increase the sensitivity. However, FECT is considered to be a dangerous method because of the use of toxic solvent in ...

Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens.

To achieve a "formalin-free laboratory" we tested straightforward-to-make fixatives along with registered reagents offered as formalin substitutes. More than two hundreds specimens were fixed in parallel with in-laboratory made fixatives PAGA (Polyethylenglycol, ethyl Alcohol, Glycerol, Acetic acid), two zinc-based fixatives (ZBF, …

A total of 800 suspension specimens including 200 samples for each technique were simultaneously and parallel processed for comparing the diagnostic …

The aim was to assess the single-vial, formalin-free fixative, Mini Parasep ® technique in comparison to the current standard methodology, i.e., formalin-ethyl acetate …

Abstract. Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered.

Aims: The objective was to compare the sedimentation spontaneous in tube technique (SSTT), the direct smear, the zinc sulfhate (surface film and sediment analyzed) methods with the formalin-ethyl ...

The formalin-ether concentration technique (FECT) is a widely used sedimentation technique for the diagnosis of intestinal protozoa in preserved stool samples . The most commonly used fixatives for stool preservation are either formalin or sodium …

Today, most people living in the endemic areas harbored the light infection. In this study, we compared the performance of formalin-ethyl acetate concentration technique (FECT), Kato-Katz technique, fecal parasite concentrator kit (FPCK) and direct simple smear technique for O. viverrini egg examination in stool.

The aim was to assess the single-vial, formalin-free fixative, Mini Parasep ® technique in comparison to the current standard methodology, i.e., formalin-ethyl acetate sedimentation technique for isolation, comparison of parasitic morphology, and to find out turnaround time for each specimen.