Abstract. The aim of this study is to investigate the effects of different fixatives on DNA, and to evaluate the fixation options for molecular studies including polymerase chain reaction (PCR) and fluorescence in-situ hybridization (FISH). Three normal-looking colonic segments from surgical resections were used for tissue sampling.

The present study documents the first investigation into the relationship between the binding and cleavage activity of nickel complexes towards DNA and proteins. Here, three different NiII complexes of the general type [Ni(nal)2diimine] (1a and 1b) and [Ni(8-Hq)2(bpy)] 1c (nal = nalidixic acid, diimine = 2,2

DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface. Such reactions may in particular impact the readout from single molecule detection studies and other ultrasensitive assays. This was highlighted by the finding that DTT markedly decreased the signal to noise ratio in a …

The effects of nickel(II) complexes with 1-allylimidazole or imidazole-4-acetate anion, and their ligands alone, on P. aeruginosa growth in correlation with pyoverdine production. White bars ...

The effects observed here on DNA synthesis were shown to be dependent on the intention of the healer with some intentions producing larger effects and others producing effects in . 3 the opposite direction. It was also demonstrated that imagery as well as intention as a …

Lingering effects of Neanderthal DNA found in modern humans. Recent scientific discoveries have shown that Neanderthal genes comprise some 1 to 4% of the genome of present-day humans whose ancestors migrated out of Africa, but the question remained open on how much those genes are still actively influencing human traits — …

Abstract. Hypoxia is a hallmark feature of the tumor microenvironment which can promote mutagenesis and instability. This increase in mutational burden occurs as a result of the downregulation of DNA repair systems. Deficits in the DNA damage response can be exploited to induce cytotoxicity and treat advanced stage cancers.

Experimental studies have shown that nickel disrupts DNA strands, DNA crosslinks, and DNA repair. Oxidative stress has been found to play a key role in nickel-induced carcinogenicity / 47, 48 /. The production of reactive oxygen species (ROS) is …

Abstract. DNA methylation is an important epigenetic mechanism for gene regulation. The conventional view of DNA methylation is that DNA methylation could disrupt protein-DNA interactions and repress gene expression. Several recent studies reported that DNA methylation could alter transcription factors (TFs) binding sequence specificity in vitro.

Studies assessing associations between prenatal exposure to antidepressants, maternal depression, and offspring DNA methylation (DNAm) have been inconsistent. Here, we investigated whether prenatal exposure to citalopram or escitalopram ((es)citalopram) and maternal depression is associated with dif …

We have directly tested the hypothesis that elevated levels of dUTP cause the formation of small DNA fragments at the replication fork of Escherichia coli. Addition of increasing levels of dUTP to lysates on cellophane discs results in an increasing number of strand scissions in the newly replicated DNA. Lysates of strains defective in dUTPase ...

The configuration of the DNA molecule is highly stable, allowing it to act as a template for the replication of new DNA molecules, as well as for the production (transcription) of the related RNA (ribonucleic acid) molecule.A segment of DNA that codes for the cell's synthesis of a specific protein is called a gene.. DNA replicates by …

Exploring the binding and cleavage activities of nickelII complexes towards DNA and proteins, M Kumar, N Lal, PM Luthra and DT Masram, New Journal of Chemistry (RSC), 2021 https: ... Neuroprotective effect of IDPU (1-(7-imino-3-propyl-2,3-dihydrothiazolo [4,5-d]pyrimidin-6(7H)-yl)urea) in 6-OHDA induced rodent model of hemiparkinson's disease, ...

There is no previous information on the use of aphidicolin in porcine embryos, so the reversible inhibition of DNA replication and the effect on embryo development of different concentrations of this drug was first evaluated. The effect of incubation with aphidicolin was tested with CRISPR/Cas9 at different concentrations and …

The effect of nickel chloride concentrations in the range of 1–1000 mg/L on seed germination and the development of organs and pigment system in seedlings of the helophyte common water-plantain Alisma plantago-aquatica L. and hygrohelophyte greater water-parsnip Sium latifolium L. has been studied. It is shown that nickel chloride has …

Biochemical experiments have shown that ncRNA binding to nucleosomal DNA is elevated at DNA entry and exit sites and is dependent on the presence of the H3 N-terminal tails. However, the structural and dynamical bases for these mechanisms remains unknown. Here, we have examined the mechanisms and effects of RDD formation in the context of …

In vivo, ncRNAs exert their influence by interacting with DNA that is packaged into chromatin fibers. The fundamental building block of chromatin is the nucleosome, a complex consisting of two copies of the H4, H3, H2A, and H2B histones, along with approximately 145–147 DNA basepairs (21).Structurally, each core histone has an …

Here we study the effect of mechanical stretching-induced structural changes on charge transport in single DNA molecules. The charge transport follows the hopping mechanism for DNA molecules with lengths varying from 6 to 26 base pairs, but the conductance is highly sensitive to mechanical stretching, showing an abrupt decrease at …

The student provides a focused and detailed description of the main topic, including a clear aim to investigate the effects of varying concentrations of nickel (II) chloride on the germination rate of Triticum aestivum. The background information provided is relevant and well-organized, covering essential concepts such as bioaccumulation and ...

Background: Tobacco smoking is suggested as a risk factor for colorectal cancer (CRC), but the complex relationship and the potential pathway are not fully understood. Methods: We performed two-sample Mendelian randomisation (MR) analyses with genetic instruments for smoking behaviours and related DNA methylation in blood …

Here we report on the chirality-driven effect of static magnetic fields (SMFs) on DNA synthesis. This newly discovered effect reveals how the interplay between two fundamental features of symmetry in living and inanimate nature-DNA chirality and the inherent features of MFs to distinguish the left and right-manifests itself in different DNA ...

In the predictor, based on the concept of the global and long-range sequence-order effects of DNA sequence, the feature called "pseudo k-tuple nucleotide composition" (PseKNC) was used to ...

Mediation analysis was subsequently performed to evaluate the potential mediating effect of DNA methylation in the association between air pollution and ceramide metabolism. PM 2.5 and O 3 were associated with elevated IR. Specifically, each 10 μg/m 3 increase in PM 2.5 and O 3 at lag0–12 h significantly increased triglyceride‑glucose ...

DNA can cause DNA damage in cancer cells, blocking the division of cancer cells and resulting in cell death.4,5 The cleavage of nucleic acids may be considered as an enzymatic

Effect of structure and composition of nickel (II) complexes with salicylidene Schiff base ligands on their DNA/protein interaction and cytotoxicity. J Inorg Biochem. 2014 …

These results suggest that nickel exposure can elevate total genomic DNA methylation levels even when DNA methyltransferase activity is depressed. These findings may explain the hypermethylation of senescence and tumor suppressor genes found during nickel carcinogenesis and support the model of a direct effect of Ni2+ on chromatin …

Introgressed Neandertal DNA has been demonstrated to significantly affect the transcriptomic landscape in people today and via this molecular mechanism influence phenotype variation as well. However, little is known about how much of that regulatory impact is mediated through long-range regulatory effects that have been shown to …

Abstract. UV radiation is selectively absorbed by DNA, mainly in the UV-B and the UV-C regions. Excitation of DNA leads to typical photoproducts, the most important being pyrimidine dimers and the so-called 6-4-photoproduct. The yield of strand breaks is very low directly after exposure, but they may be formed indirectly upon further incubation.

Docking studies on DNA complexes confirm the binding of 1 and 2 in the major groove of CT-DNA (CTP-1 Endonuclease). Moreover, the antibacterial effect of 1-3 against the five bacterial species was ...

The DNA binding capacity values measured in this study were lower compared to published spiking studies on AEX chromatography membranes using commercial herring sperm DNA (hs‐DNA).11, 12 The differences could be attributed to several factors including operational conditions and the different types of DNA used for …

The nuclear insertion of organellar sequences can occur during the illegitimate repair of double-stranded breaks. After integration, nuclear organelle DNA is modified by point mutations, and by deletions. Insertion of organelle DNA into nuclear genes is not rare and can potentially have harmful effects. In humans, some insertions of nuclear ...

In DNA repair, NER is the major mechanism for the repair of conventional bulky lesions. NER exhibits a robust activity for DNA–peptide cross-links, but a poor to negligible activity for DPCs. The initial recognition of DPCs by NER factors appears to be critical, and is compromised due to the steric hindrance of DPCs.

After DNA damage occurs, cells activate several response signals, such as cell-cycle arrest, DNA repair, and cell death [ 26, 27, 28 ]. Numbers in in vitro and in …

In this paper we used a force-measuring optical trap instrument to determine the elastic properties of λ DNA as a function of ionic strength and in the presence of multivalent cations.Our results show the following effects, some of them unexpected, of ion concentration, valence, and structure on DNA elasticity: (i) The electrostatic contribution …

One of these endogenous danger signals is the extracellular mitochondrial DNA (mtDNA) containing evolutionary conserved unmethylated CpG repeats. ... (HLA-DQ) on the cell surface, as well as increased TNF-α and IL-8 production from the cells. These effects were more apparent when pDCs were exposed to oxidatively modified mtDNA. Neither native ...

Effect of nickel (II) on the specific binding of proteins to UV- irradiated DNA and protective interaction of magnesium (II). HeLa cells were incubated for 24 h with the respective...

We propose a model in which changes in unconstrained DNA supercoiling influences higher levels of chromatin organisation through the additive effects of DNA supercoiling on both DNA-protein and DNA-nucleosome interactions. This model links small-scale changes in DNA and chromatin to the higher-order fibre and large-scale chromatin structures ...